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cialis cost In addition, HEK293T cells were transfected with plasmid constructs expressing the mutants of RabGAP1L N 3xHA RabGAP1L О” PTB RabGAP1L with deletion of residues 1 471 defining the PTB domain and N 3xHA RabGAP1L О” TBC RabGAP1L with deletion of residues 585 815 comprising the TBC domain in pEZ M06, see molecular cloning, Supplementary Data 6